We narrowed to 17,800 results for: Jun
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Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Golgi-7
Plasmid#57959PurposeLocalization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565DepositorInsertGolgi (B4GALT1 Human)
TagsmOrange2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
Plasmid#90014PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6CDepositorInsertsCBirA*-Ago2
NBirA*-TNRC6C
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-NLS-Beta-catenin-S33Y
Plasmid#138537PurposeFor making Beta-catenin-S33Y RNADepositorInsertBeta-catenin
UseIn vitro transcriptionTags2XFLAG and 2xNLSMutationchanged serine 33 to tyrosinePromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxExpressionMammalianAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD9 Flag
Plasmid#74901PurposeExpress COMMD9 in mammalian cellsDepositorAvailable SinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD21-hgAPOAI
Plasmid#124962PurposeExpress ApoAI in the liverDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
ExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3a
Plasmid#111055PurposeExpresses dLight1.3a in mammalian cellsDepositorInsertdLight1.3a
TagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL437 AAVS1 EQR sgRNA
Plasmid#126447PurposeTo cut the human AAVS1 locus for integration.DepositorInsertspacer against human AAVS1 safe-harbor locus with NGAG PAM
ExpressionMammalianPromoterhuman U6Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(NC)-SH2
Plasmid#46521DepositorInsertSyk(NC) (SYK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-263PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC297 VEGF Site#1
Plasmid#47505Purposehuman gRNA expression vector targeting VEGFDepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
Plasmid#90012PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Cdc25C
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-S259A
Plasmid#31728DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only