We narrowed to 11,713 results for: NSI

1. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

   Plasmid

   #154769

   Purpose

   plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

   Plasmid

   #145268

   Purpose

   Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2(+10A)-IDR (RUNX2 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiRCas9-CUG

   Plasmid

   #104183

   Purpose

   Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

   Depositor

   Eugene Yeo

   Article

   Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

   Insert

   U6-CUGsgRNA, EFS-PIN-dCas9
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3HAT1Luc1

   Plasmid

   #236275

   Purpose

   Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

   Insert

   angiotensin II type 1 receptor (AGTR1 Human)
   

   Tags

   3xHA and Rluc8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Available Since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PB-CAG-ChR2-YFP

   Plasmid

   #204345

   Purpose

   PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   ChR2-YFP
   

   Tags

   Yellow fluorescent protein

   Expression

   Mammalian

   Mutation

   C-terminus (aa315-737) is deleted - PMID: 1461559…

   Promoter

   CAG

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nsp14-EGFP

   Plasmid

   #165119

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6_YAP1-V5

   Plasmid

   #173002

   Purpose

   constitutive expression of V5-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   YAP1-V5 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_UCN1.0

   Plasmid

   #208669

   Purpose

   Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T-U6-sgGAA

    Plasmid

    #232724

    Purpose

    Cas9 guide RNA targeting GAA repeats for A-to-G base editing

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    SpCas9 gRNA targeting GAA repeats
    

    Expression

    Mammalian

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Plasmid

    #159580

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TVBB C-term-mNeongreen

    Plasmid

    #169227

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo-RL-ZEB2 200bmut

    Plasmid

    #35538

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB2 gene (ZEB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    6 Mutations in the miR-200b seed regions of the Z…

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_BTK-1/1

    Plasmid

    #91350

    Purpose

    Protein expression and purification of human SH3 domain construct BTK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BTK-1/1 (BTK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_GRB2-1/2

    Plasmid

    #91480

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-1/2 (GRB2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetO-hcMYC

    Plasmid

    #206387

    Purpose

    Expresses human c-MYC in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    c-MYC (MYC Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV7.1_3xFLAG-WWC1

    Plasmid

    #172994

    Purpose

    constitutive expression of FLAG-tagged WWC1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC1 (WWC1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE2-TetO-hSOX17FNV

    Plasmid

    #206384

    Purpose

    Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    SOX17FNV (SOX17 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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