We narrowed to 11,713 results for: NSI

1. pcDNA3/hArf5(WT)-mCherry

   Plasmid

   #79421

   Purpose

   Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf5 (ARF5 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp10-mCherry

   Plasmid

   #165135

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp10 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

   Plasmid

   #176250

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR and Synthetic Biology; Expression in micro…

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-oROS-HT

   Plasmid

   #216416

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   pDisplay-oROS-HT
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. OA-1136J

   Plasmid

   #153023

   Purpose

   Express His-MBP-TEV-CasRx recombinant protein

   Depositor

   Omar Akbari

   Article

   Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

   Insert

   CasRx
   

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MmEos3.2

   Plasmid

   #203754

   Purpose

   Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Src15 n-term (SRC Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_ITK-1/1

   Plasmid

   #91253

   Purpose

   Protein expression and purification of human SH3 domain construct ITK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITK-1/1 (ITK Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL8 lenti-D10Acas9-3xflag-blast

   Plasmid

   #112134

   Purpose

   lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   D10Acas9-3xflag-blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A mutant in Cas9

   Promoter

   EF1a

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XFL-Src-S4-UD-mCherry

   Plasmid

   #140306

   Purpose

   Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X FL

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions fused to mCh…

   Promoter

   CMV

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-GRAB_UCN1.0

    Plasmid

    #208667

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC_hfnCas12a-Nlux

    Plasmid

    #176239

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Promoter

    Ribosomal subunit

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-S4-UD-S32

    Plasmid

    #140303

    Purpose

    Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-mCherry-hCdt1(1-100)ΔCy

    Plasmid

    #193759

    Purpose

    Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
    

    Use

    Lentiviral

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PXPR007 sgCIC-2

    Plasmid

    #74959

    Purpose

    Cas9 + sgCIC-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP-CMV-puro-2A-BLM

    Plasmid

    #127641

    Purpose

    Over-expression of human BLM

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    BLM (BLM Human)
    

    Use

    Lentiviral

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nsp7-EGFP

    Plasmid

    #165111

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-1 Flag-LplA

    Plasmid

    #232118

    Purpose

    For bacterial expression of Flag-tagged E. coli LplA.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    lplA (lplA Escherichia coli (strain K12))
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. TVBB N-term-mScarlet

    Plasmid

    #169218

    Purpose

    Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-GRAB_VIP1.0

    Plasmid

    #208682

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_CCK1.0

    Plasmid

    #208672

    Purpose

    Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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