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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

Plasmid

#62957

Purpose

Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4 in three phases

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -1

Plasmid

#173887

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

Plasmid

#193853

Purpose

Promoterless insertion vector for split HygR/mScarlet-I landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

5' rps-0::HygR + 5' mScarlet-I

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

rps-0p

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-HXGPRT-LoxP

Plasmid

#86553

Purpose

used for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondii

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-DHFR-LoxP

Plasmid

#86669

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

promoterless

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGE-attBTT-loxP-DsRed (pTL780)

Plasmid

#115160

Purpose

Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

attB-TT loxP 3xP3-DsRed

Use

Cre/Lox

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSL24a-Linker-smHA-FP-DHFR-LoxP

Plasmid

#166238

Purpose

Tagging of weakly expressed genes, floxed DHFR marker

Depositor

David Sibley

Insert

Spaghetti monster HA

Use

Tags

Expression

Mutation

none

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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