We narrowed to 2,514 results for: organization Addgene

1. mCherry-miniSOG-Tubulin-6

   Plasmid

   #55097

   Purpose

   Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

   Plasmid

   #112842

   Purpose

   transient/stable overexpression of the central domain of Catulin.

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Catulin-alpha-1 (Ctnnal1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C1 domain of Catulin

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

   Plasmid

   #227158

   Purpose

   For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   cyp-33e1 (cyp-33E1 Nematode)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 295 and cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227163

   Purpose

   For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 82 and cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227164

   Purpose

   For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 14, cysteine 82, and cysteine 43…

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+8-PCSK1-flag-tev-halo-his

   Plasmid

   #227155

   Purpose

   For T-REX expriments of PCSK1.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   PCSK1 (PCSK1 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-Myc-BirA*G3

   Plasmid

   #240233

   Purpose

   Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   Myc-BirA*G3
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT-sfGFP-TurboID-ER

   Plasmid

   #240230

   Purpose

   Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   BIP-sfGFP-TurboID-KDEL
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT-sfGFP-TurboID

   Plasmid

   #240231

   Purpose

   Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   sfGFP-TurboID
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAS-sfGFP-TurboID-ER

    Plasmid

    #240232

    Purpose

    Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BIP-sfGFP-TurboID-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT-Myc-BirA*G3-ER

    Plasmid

    #240234

    Purpose

    Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BiP-Myc-BirA*G3-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUAS-CG6867-sfGFP

    Plasmid

    #240237

    Purpose

    Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    CG6867 (CG6867 Fly)
    

    Use

    Tags

    sfGFP

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWal10-roe-sfGFP

    Plasmid

    #240239

    Purpose

    Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR_BIP-sfGFP-TurboID-KDEL

    Plasmid

    #240223

    Purpose

    Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BIP-sfGFP-TurboID-KDEL
    

    Use

    Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR_spatzle-HA

    Plasmid

    #240227

    Purpose

    Gateway entry clone with spatzle tagged with HA (contains stop codon)

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    spatzle (spz Fly)
    

    Use

    Gateway shuttle vector

    Tags

    HA-spGFP11

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tg(Sox17::EMTB-3xGFP)

    Plasmid

    #221190

    Purpose

    To specifically label microtubules (MTs) in Kupffer's Vesicle (KV)

    Depositor

    Heidi Hehnly

    Article

    Wu et al Development. 2025 May 15;152(10):dev2046

    Insert

    Ensconsin Microtubule-Binding Protein (MAP7 Human)
    

    Use

    Zebrafish

    Tags

    3 EGFP

    Expression

    Mutation

    Microtubule binding-domain of ensconsin (A.A. 18…

    Promoter

    Sox17

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

    Plasmid

    #225353

    Purpose

    Lentiviral vector for DKK1 and BFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    Dkk1 (Dkk1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

    Plasmid

    #225350

    Purpose

    piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    H2B (H2B Human, Synthetic)
    

    Use

    Tags

    miRFP and non-fluorescent GFP (Y67F)-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-Ctr1-gRNA-EGFP

    Plasmid

    #226000

    Purpose

    Negative control for CRISPRi-KD

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    negtive control sgRNA of no specific targets in human genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-Ctr2-gRNA-EGFP

    Plasmid

    #226001

    Purpose

    Negative control for CRISPRi-KD

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    negtive control sgRNA of no specific targets in human genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge