pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid
#196082
Purpose
(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
Depositor
Inserts
dCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
Use
CRISPR
Tags
Expression
Mammalian
Mutation
Catalytically dead mutant of the Cas9 endonucleas…
Promoter
Available since
March 30, 2023
Availability
Academic Institutions and Nonprofits only