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pcDNA3.1-VcnTS-E1021A

Plasmid

#213416

Purpose

Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1021 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnCS-E1015A

Plasmid

#213412

Purpose

Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnCS-E1015A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1015 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnCS-E1021A

Plasmid

#213413

Purpose

Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnCS-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1021 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBS CRABP2

Plasmid

#19188

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Cellular retinoic acid binding protein 2 (CRABP1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBS CLN8

Plasmid

#19212

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

CLN8 (CLN8 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS MIP2gamma

Plasmid

#19224

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Jun-suppressed chemokine (CXCL14 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS ABI3BP

Plasmid

#19191

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

ABI3BP (ABI3BP Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pC0.066

Plasmid

#119666

Purpose

Level 0 part. Terminator from pheA gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TpheA-1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Control

Plasmid

#127582

Purpose

Expresses 5S-F30-Control rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-TtCas6-4xcrRNA4.5

Plasmid

#127764

Purpose

For expression of T. thermophilus Csm complex in E. coli (crRNA)

Depositor

Jennifer Doudna

Article

Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

Inserts

Synthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli

Promoter

T7

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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iTol2Amp-γ-crystallin:RFP

Plasmid

#108455

Purpose

This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

Tol2-Amp-y-cryst-mCherry

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Desmoglein2_tail-GST

Plasmid

#36990

Depositor

Kathleen Green

Article

Brennan et al Oncogene. 2012 Mar 29;31(13):1636-48. do

Insert

Dsg2 (DSG2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Intracellular domain of DSG2 (nucleotides 1911–35…

Promoter

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
pAPM-miR30-XPO1-ts1

Plasmid

#174286

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-XPO1-ts2

Plasmid

#174287

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-XPO1-ts3

Plasmid

#174288

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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HySp5-420

Plasmid

#192993

Purpose

Plasmid that encodes for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-Paxillin-22

Plasmid

#54935

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Paxillin

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Use

Tags

Expression

Plant

Mutation

Promoter

FRO6

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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