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Plasmid#207409PurposeProtein purification vector to produce AcrIC9 in E. coliDepositorInsertT7-TEV-AcrIC9
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-3C-AcrIC8
Plasmid#207410PurposeProtein purification vector to produce AcrIC8 in E. coliDepositorInsertT7-HRV3C-AcrIC8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAXY0018
Plasmid#205587Purposep3flag_HygR(1-52)-NpuDnaE(N)DepositorInsertHygR-NpuDnaE(N)_homo-codon-OP
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT4 (MT4 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJ4M_HRP-1_FL
Plasmid#139117Purposeexpress HRP-1 with a C-terminal maltose binding protein tagDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3
Plasmid#133343Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_gcrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA
Plasmid#133345Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_blaA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-YbATP-grasp
Plasmid#129639PurposeExpression plasmid of ATP-grasp from Y. bercovieriDepositorInsertYbATP-grasp
UseTagsHis6-tagExpressionBacterialMutationPromoterpQEAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaTPR mutant
Plasmid#67614Purposebacterial expression of GST-PRMT9 missing residues 21-139DepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationtruncation mutant missing aa 21-139PromotertacAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaN (aa 21-350)
Plasmid#67611Purposebacterial expression of GST-PRMT9 missing residues 21-350DepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationtruncation mutant missing aa 21-350PromotertacAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ADAM21i1
Plasmid#59604PurposeExpression of shRNA against human ADAM21DepositorInsertADAM21 (ADAM21 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 pET15b
Plasmid#60679PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationM26LPromoterT7Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-SLC17A5i1
Plasmid#59598PurposeExpression of shRNA against human SLC17A5DepositorInsertSLC17A5 (SLC17A5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-PTPRJi2
Plasmid#59609PurposeExpression of shRNA against human PTPRJDepositorInsertPTPRJ (PTPRJ Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only