We narrowed to 2,598 results for: pCas
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Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-SpCas9_sgRNA-site2 (RTW448)
Plasmid#160137PurposeT7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2DepositorInsertSpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
UseIn vitro transcription of sgrna from t7 promoterPromoterT7Available sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCASP-SptP120-E3_5-HilA
Plasmid#153326PurposeTriggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-E3_5 DARPin
UseSalmonella expressionAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF1-2A-EGFP-L2
Plasmid#141048PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF1-2A-EGFP
ExpressionMammalianAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-eSpCas9-plus
Plasmid#126774PurposeExpression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.DepositorInserteSpCas9-plus
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A, R1060A; amino acids 1005-1013 replaced wit…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone32 (Opti-SpCas9)
Plasmid#131736PurposeExpresses Opti-SpCas9 in mammalian cellsDepositorInsertOpti-SpCas9
UseLentiviralTagsFlagMutationR661A+K1003HPromoterEFSAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-Flag-eSpCas9-H840A
Plasmid#80454Purposeenhanced SpCas9 mammalian expression vectorDepositorInsertehSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9 UGT1 (pWZ594)
Plasmid#254037PurposeExpress SpCas9 and the UGT1 gRNADepositorInsertgRNA_UGT1
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available sinceMay 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 LP400~3p (pKBA314)
Plasmid#254039PurposeExpress SpCas9 and the LP400~3p gRNADepositorInsertgRNA_LP400~3p
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available sinceMay 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 UNS1 (pKBA244)
Plasmid#254040PurposeExpress SpCas9 and the UNS1 gRNADepositorInsertgRNA_UNS1
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available sinceMay 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 UNS2 (pKBA235)
Plasmid#254041PurposeExpress SpCas9 and the UNS2 gRNADepositorInsertgRNA_UNS2
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available sinceMay 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX461-ABE8e-nSpCas9
Plasmid#237460PurposeTransfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.DepositorInsert3xFlag_ABE8e_SpCas9(D10A)_6xNLS
UseCRISPRTags3xFLAGExpressionMammalianMutationD10APromoterchicken beta-actin promoter & U6Available sinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX461-evoCDA1-nSpCas9
Plasmid#237461PurposeTransfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.DepositorInsert3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
UseCRISPRTags3xFLAGExpressionMammalianMutationD10APromoterchicken beta-actin promoter & U6Available sinceJan. 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-spCas9
Plasmid#231403PurposeExpresses spCas9 from GFAP promoterDepositorAvailable sinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-SpCas9
Plasmid#214603Purposeencode SpCas9; in vitro transcribed modified RNA vectorDepositorInsertSpCas9
UseCRISPR and UnspecifiedPromoterT7Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-SMCR8
Plasmid#83440PurposeExpresses Cas9 and a gRNA targeting SMCR8DepositorAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only