166,289 results

1. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-syn-ChR2-EYFP-Kv

   Plasmid

   #89256

   Purpose

   Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

   Depositor

   McLean Bolton

   Article

   Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

   Insert

   Channelrhodopsin
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   synapsin

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p189

   Plasmid

   #65207

   Purpose

   Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Expression

   Bacterial and Mammalian

   Mutation

   Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAWP89

   Plasmid

   #61264

   Purpose

   IncP-based replicating vector expressing dTomato under tac promoter.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Insert

   Ptac-dTomato
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEM5Z(+)-EGFP

   Plasmid

   #65206

   Purpose

   EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Expression

   Bacterial and Mammalian

   Mutation

   Restriction enzyme recognition sites added either…

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Expression

   Bacterial

   Promoter

   AmpR and lpp

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3/Myc-DNMT1

   Plasmid

   #36939

   Purpose

   Mammalian expression of human DNMT1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

   Insert

   DNMT1 (DNMT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156643

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5A (KIR2DL5A Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti6.2_mNeonGreen2

   Plasmid

   #113727

   Purpose

   Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Article

   Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

   Insert

   mNeonGreen2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-EZH2

    Plasmid

    #116738

    Purpose

    Lentiviral expression of EZH2

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge