We narrowed to 12,028 results for: LEA

Showing: 7581 - 7600 of 12028 results

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pLENTICRISPR V2 hygro sgmPOLE_2

Plasmid

#241443

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_2 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_1

Plasmid

#241442

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_1 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgRELB_1

Plasmid

#241452

Purpose

Delete RELB

Depositor

Richard Possemato

Insert

sgRELB_1 (RELB Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2-mCherry sgmRELA_1

Plasmid

#241447

Purpose

Delete mouse Rela

Depositor

Richard Possemato

Insert

sgmRELA_1 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 Hygro sgRELA_4

Plasmid

#241446

Purpose

Delete RELA

Depositor

Richard Possemato

Insert

sgRELA_4 (RELA Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A/E64M

Plasmid

#231521

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutations at position 61 Q to A and 64 E to…

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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hTln1-R12DD-SCLS

Plasmid

#191443

Purpose

expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

Depositor

Ben Goult

Article

Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

Insert

Human Talin1 R12DD (TLN1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

deletion between residues K2375 and A2397

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET24TEV-tSHIP2

Plasmid

#183771

Purpose

Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Tags

6HIS and Tobacco Etch Virus (TEV) Cleavage site

Expression

Bacterial

Mutation

includes aa18 to aa985 only

Promoter

T7 lac

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TtNAM-B1

Plasmid

#124575

Purpose

TtNAM-B1 CDS in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-B1

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TaNAM-A1(1-217)

Plasmid

#124576

Purpose

NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1 (1-217)

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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p3.2mar(T->G)-Luc

Plasmid

#63697

Purpose

3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expression

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change

Tags

Luciferase

Expression

Mammalian

Promoter

TATA box

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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