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pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only
pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB107

Plasmid

#68561

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA

Plasmid

#106113

Purpose

Lentiviral expression of PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLG027 (qatABCD)

Plasmid

#157905

Purpose

Expresses the qatABCD defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB102

Plasmid

#68556

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLIC-SM-HA

Plasmid

#111188

Purpose

High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

Depositor

Silvia Moreno

Article

Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

Type

Empty backbone

Use

Toxoplasma gondii expression

Tags

Spaghetti Monster HA Tag

Promoter

Endogenous

Available Since

Aug. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-Myc-gate-pGK-HYG

Plasmid

#107395

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

Myc

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Tags

YFP

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Ebp1

Plasmid

#67792

Purpose

Mammalian expression of Ebp1 tagged with HA

Depositor

Stephen Goff

Article

Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

Insert

Ebp1 (PA2G4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pTECH-MbPylRS(IPYE)

Plasmid

#104072

Purpose

Expression plasmid encoding MbPylRS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MbPylRS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLIC-SM-Myc

Plasmid

#111189

Purpose

High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

Depositor

Silvia Moreno

Article

Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

Type

Empty backbone

Use

Toxoplasma gondii expression

Tags

Spaghetti Monster Myc Tag

Promoter

Endogenous

Available Since

Aug. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMSR0

Plasmid

#161984

Purpose

Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

Depositor

Olga Soutourina

Article

Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 11, 2021

Availability

Academic Institutions and Nonprofits only

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