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FLAG-SLC30A10-E25A

Plasmid

#82345

Purpose

Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

E25A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-8

Plasmid

#172761

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-5

Plasmid

#172760

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-5

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR2C2_Hormone-recep

Plasmid

#109837

Purpose

Protein expression and purification of NR2C2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2C2_Hormone-recep (NR2C2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

Plasmid

#182345

Purpose

GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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MTK3_001

Plasmid

#123952

Purpose

Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3XNLS::CRY2PHR::p65

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_5

Plasmid

#91170

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_mTLN1-FL-LBD

Plasmid

#164839

Purpose

Expression of GFP tagged FL mouse talin1 mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His8-GFP

Expression

Mammalian

Mutation

Lipid binding deficient mutations

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Peroxisomes-2

Plasmid

#54804

Purpose

Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

mCherry-miniSOG-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pIW086

Plasmid

#134132

Purpose

Bacterial expression of His-TEV-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-TEV-human Ska1 ; human Ska2

Tags

His-TEV

Expression

Bacterial

Mutation

K183A K184A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM 3B-His

Plasmid

#172192

Purpose

Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

hFAM 3B

Tags

His

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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ZJOM12

Plasmid

#133638

Purpose

Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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5mCUC1 pGreenII0129

Plasmid

#12067

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJL2

Plasmid

#184130

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#184129

Purpose

For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1 (3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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