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pBAD-ColH-HylB

Plasmid

#248631

Purpose

expresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coli

Depositor

Edwin Manuel

Article

Avsharian et al Biomolecules. 2024 Nov 17;14(11):1458. d

Insert

Myc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB

Tags

His and Myc

Expression

Bacterial

Promoter

araBAD

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TPC2-mCherry

Plasmid

#240468

Purpose

Red-tagged lysosomal channel

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Mutated aspartate 276 to lysine.

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-rNSE-AcGFPnuc-WPRE

Plasmid

#245088

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Rat NSE promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Rat NSE promoter

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-mPGK-AcGFPnuc-WPRE

Plasmid

#245090

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Mouse PGK promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Mouse PGK promoter

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-mGAD67-AcGFPnuc-WPRE

Plasmid

#245086

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Mouse GAD67 promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Mouse GAD67 promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-mD1R promoter-AcGFPnuc-WPRE

Plasmid

#245087

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Mouse D1R promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Mouse D1R promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-rTAC1-AcGFPnuc-WPRE

Plasmid

#245089

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Rat TAC1 promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Rat TAC1 promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SIN-GluR1-AcGFPnuc-WPRE

Plasmid

#245092

Purpose

Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Rat GluR1 promoter

Depositor

Nicole Déglon

Article

Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

Insert

AcGFP

Use

Lentiviral

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

Rat GluR1 promoter

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Plasmid

#187078

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMJJ4-FOXO1-IRES-Thy1.1

Plasmid

#132702

Purpose

Expresses Thy1.1 and FOXO1

Depositor

Mandy Ford

Article

Krummey et al iScience. 2020 Apr 24;23(4):100912. doi:

Insert

FOXO1 (FOXO1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMJJ4-FOXO3-IRES-Thy1.1

Plasmid

#132703

Purpose

Expresses Thy1.1 and FOXO3

Depositor

Mandy Ford

Article

Krummey et al iScience. 2020 Apr 24;23(4):100912. doi:

Insert

FOXO3 (FOXO3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-iS-methadoneSnFR-Golgi

Plasmid

#220865

Purpose

Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHHM.X513.iS-methadoneSnFR

Plasmid

#220861

Purpose

Bacterial expression of iS-methadoneSnFR for purification and in vitro assays.

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Tags

His6-HA and Myc

Expression

Bacterial

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_212

Plasmid

#210778

Purpose

C2_Pyrearinus termitilluminans click beetle luciferase ('Eluc' version - human-codon optimized)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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NLuc-His10

Plasmid

#234567

Purpose

Bacterially expressed NLuc for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Nano luciferase

Tags

T7 gene 10 leader peptide and Thrombin site-10xHi…

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

T7

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Esyn-FlpX-TVA950-EYFP-WPRE

Plasmid

#234710

Purpose

pAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFP

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

TVA950-eYFP

Use

AAV; Has fdio cassette for flp-dependent expressi…

Expression

Mammalian

Promoter

enhanced Synapsin

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA047_Podxl

Plasmid

#232932

Purpose

Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Podxl S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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