We narrowed to 11,806 results for: nsf

1. pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

   Plasmid

   #213034

   Purpose

   fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

   Insert

   mNeonGreen, ISN-IE, ChRmine
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Promoter

   hSyn

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SMVP-Cas9N-U6-gRNA TdR

   Plasmid

   #80940

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113760

   Purpose

   AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   Synapsin

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM55297

   Plasmid

   #153213

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

   Plasmid

   #39455

   Depositor

   Hongjun Song

   Article

   Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

   Insert

   HA-hTet1CDmu (TET1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1418–2136 with mutations H1672 to Y, 1674D to A

   Promoter

   EF1a

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pMK-Cas9-gate

   Plasmid

   #113743

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCHR-inmCherry

   Plasmid

   #60239

   Purpose

   HIV-1 transfer vector that encodes mcherry reporter gene interrupted by intron

   Depositor

   Dmitriy Mazurov

   Article

   Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

   Insert

   inmCherry
   

   Use

   Lentiviral

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Promoter

   EF1a

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-FlicR1

    Plasmid

    #74143

    Purpose

    Express FlicR1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hRedOpsin-mCherry

    Plasmid

    #164426

    Purpose

    AAV plasmid expressing mCherry in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human red opsin

    Available Since

    Nov. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-pAceR

    Plasmid

    #195521

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKII-GRAB_eCB2.0

    Plasmid

    #164608

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    CaMKII

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-mDLX-TVA-2A-N2cG

    Plasmid

    #172364

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDLX

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. aav-tnt-dnmrtfa-gfp

    Plasmid

    #165035

    Purpose

    AAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivo

    Depositor

    William Pu

    Article

    Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    dnMRTFA
    

    Use

    AAV

    Tags

    GFP

    Mutation

    deletion of 100 N-terminal amino acids

    Promoter

    cTNT

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

    Plasmid

    #51473

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6m
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. penaav-tnt-3xha-p2a-gfp

    Plasmid

    #175090

    Purpose

    For making AAV9

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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