We narrowed to 13,228 results for: sequence

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C513-E06: attB5r-pA-polyhedrinp>-attB1r

Plasmid

#162948

Purpose

Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter with upstream polyA sequence

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3b

Plasmid

#166054

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3b

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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PaCasp3

Plasmid

#166052

Purpose

a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Porites astreiodes caspase-3

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

Plasmid

#163927

Purpose

Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer

Plasmid

#138365

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161k human enhancer

Use

Gateway compatible

Mutation

9 A bases deletion, that have no impact on the en…

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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phNFL-L268P

Plasmid

#132593

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E L268P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

803T>C; Leu268Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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phNFL-P8R

Plasmid

#132590

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

Plasmid

#133330

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All FYW to L in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

Plasmid

#118812

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

W385G in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]

Plasmid

#118814

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G295F in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]

Plasmid

#118805

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of RRM in full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]

Plasmid

#118806

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of CTD in full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1385 TDP43 RRM-GFP VLIM-S

Plasmid

#118792

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All VLIM to S in CTD of TDP43 RRM-GFP reporter

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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