We narrowed to 49,050 results for: prot

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pNH-TrxT-G-S15

Plasmid

#127823

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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BAS290 C terminal E2C natR

Plasmid

#74085

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pSPVpr-hp

Plasmid

#48119

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of Vpr

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Opto-FFR3

Plasmid

#106065

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-FFR3

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Shp2 SH2s

Plasmid

#67575

Purpose

Expresses GST-tagged Shp2 SH2 domains in bacteria

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2 SH2s

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acids 219-597

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330) S278C

Plasmid

#87739

Purpose

Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-277, mutated Ser 278 to Cys

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-FRB-Venus-173-N

Plasmid

#87615

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-Venus173-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(81-437)

Plasmid

#87734

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-80

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDonr201 RNF146

Plasmid

#240330

Purpose

Entry vector for Gateway with RNF146

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTARGEX-ER

Plasmid

#242449

Purpose

Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.

Depositor

Allyson MacLean

Article

Chartrand-Pleau et al Front. Synth. Biol. 3:1619871

Type

Empty backbone

Tags

ER-retrieval motif (KDEL) and Signal peptide deri…

Expression

Plant

Promoter

double 35S

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLP110_AAV Scramble Control

Plasmid

#239419

Purpose

Negative control AAV vector for SaCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble control

Use

AAV

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Av2

Plasmid

#125642

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Bv2

Plasmid

#125643

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-STE3-GFP

Plasmid

#221113

Purpose

GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

STE3-GFP

Expression

Yeast

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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