Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,666 results for: try

Clear keywords
Showing: 7621 - 7640 of 7666 results

  1. pLKO_V5.AU1.C_NGFR

    Plasmid
    #158255
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.AU1.C
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158255-plasmid-map-sequence-id-327854

    Enlarge

  2. pLKO_VSVg.AU1.C_NGFR

    Plasmid
    #158256
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    VSVg.AU1.C
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158256-plasmid-map-sequence-id-334984

    Enlarge

  3. pLKO_FLAG.V5.C_NGFR

    Plasmid
    #158259
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.V5.C
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158259-plasmid-map-sequence-id-327882

    Enlarge

  4. pLKO_HA.AU1.NWS_NGFR

    Plasmid
    #158234
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.NWS
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158234-plasmid-map-sequence-id-334982

    Enlarge

  5. pLKO_HA.AU1.FLAG_NGFR

    Plasmid
    #158236
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158236-plasmid-map-sequence-id-334983

    Enlarge

  6. pLKO_NWS.AU1.V5_NGFR

    Plasmid
    #158237
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.V5
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158237-plasmid-map-sequence-id-327677

    Enlarge

  16. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

    Enlarge

  17. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72924
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Gateway Cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72924-plasmid-map-sequence-id-138470

    Enlarge

  18. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

    Enlarge

  20. pZB246-T7-T+

    Plasmid
    #250903
    Purpose
    Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone
    Depositor
    Timothy Whitehead
    Article
    Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.
    Insert
    Thermally Stable T7 RNA Polymerase
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Arginine 31 to Aspartic Acid, Alanine 61 to Argin…
    Promoter
    pT5-lacUV
    Available Since
    Feb. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250903-plasmid-map-sequence-id-499452

    Enlarge

Showing: 7621 - 7640 of 7666 results