We narrowed to 8,503 results for: Set

1. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Lee Rubin, Tim Ahfeldt

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Use

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv2.1 K+ channel scFv [K39/25]

   Plasmid

   #182067

   Purpose

   Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GABA(A)R, Alpha1 scFv [N95/35]

   Plasmid

   #199430

   Purpose

   Mammalian expression of GABA(A)R, Alpha1 scFv. Derived from hybridoma N95/35.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targetingGABA(A)R, Alpha1 (Human) (GABRA1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kv4.2 K+ channel (external) scFv [K57/41]

   Plasmid

   #206771

   Purpose

   Mammalian Expression of Kv4.2 K+ channel (external) scFV. Derived from hybridoma K57/41 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv4.2 K+ channel (external) (Homo sapiens) recombinant scFV (KCND2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GABA(A)R, Gamma2L/S scFv [N452/73]

   Plasmid

   #190555

   Purpose

   Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-hSyn-PROGERIN

    Plasmid

    #140189

    Purpose

    Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    Progerin (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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