We narrowed to 14,009 results for: flag

1. KLC1-Myc

   Plasmid

   #166962

   Purpose

   Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1 (Klc1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB-hCRY1-Luciferase-hvTK-bla

   Plasmid

   #179444

   Purpose

   Donor vector to knock in firefly Luciferase C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   Luciferase
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF1-MCS2-EF1-Puro-RGS7

   Plasmid

   #113728

   Purpose

   Express RGS7 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VQRRA-P2A-Puro

   Plasmid

   #110851

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB-hCRY1-SNAP-CD4-bla

   Plasmid

   #179447

   Purpose

   Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   SnapTag
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CHAF1B_pLENTI-CAG-IRES-GFP

   Plasmid

   #177009

   Purpose

   Mammalian lentiviral expression vector encoding CHAF1B

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   CHAF1B (CHAF1B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-F-Gcn5-NLSm

   Plasmid

   #63708

   Purpose

   retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated K425, K427 and K438 to R (arginine)

   Promoter

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCAT526

   Plasmid

   #180513

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv1
   PlmCasX-2A-mNeonGreen
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGEX-DDX21-D2+C

   Plasmid

   #179243

   Purpose

   protein purification in E. coli

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21-D2+C (DDX21 Human)
   

   Use

   Tags

   GST-flag

   Expression

   Bacterial

   Mutation

   aa396-782

   Promoter

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL472

    Plasmid

    #126457

    Purpose

    Expresses Cas9-10xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    10x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid

    #129794

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G102H

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. KRAS-V14I

    Plasmid

    #154427

    Purpose

    V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

    Depositor

    Kenneth Westover

    Article

    Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCDF1-GRM3 (E870K)

    Plasmid

    #31802

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 870 to lysine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDF1-GRM3 (E767K)

    Plasmid

    #31801

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 767 to lysine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pCDF1-GRM3 (G561E)

    Plasmid

    #31799

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glycine 561 to glutamic acid

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pLenti-VRERRA-PGK-Puro

    Plasmid

    #110859

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-VRERRA-P2A-Puro

    Plasmid

    #110852

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-NSD3-short del(100-263)

    Plasmid

    #72548

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3xFlag and SV40 NLS

    Expression

    Mammalian

    Mutation

    100-263 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA5-F-Med26-K74A K75A

    Plasmid

    #49253

    Purpose

    Mammalian expression of flag-tagged human Med26-K74A K75A

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K74A K75A

    Promoter

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pCIN4-EZOD

    Plasmid

    #44977

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 DBD (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Ets2 DNA binding domain (AA 332-468) only = domin…

    Promoter

    CMV

    Available Since

    May 28, 2013

    Availability

    Academic Institutions and Nonprofits only

27. CtkA_300_cmv-10

    Plasmid

    #27434

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    residues 301-325 are truncated

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

28. CtkA_D155Q/D179Q_cmv-10

    Plasmid

    #27435

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D155Q/D179Q

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235307

    Purpose

    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235308

    Purpose

    Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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