166,289 results

1. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ10530

   Plasmid

   #239266

   Purpose

   Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   NES-dPspCas13b-EGFP-ABI
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag tag and V5 tag

   Expression

   Mammalian

   Mutation

   H133A and H1058A in PspCas13b

   Promoter

   EF1a

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138132

   Purpose

   Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-mouse-IgG(kappa)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DIO-ATLASsnFlp

   Plasmid

   #232352

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Has Service

   AAV8

   Insert

   ATLASsnFlp
   

   Use

   AAV

   Tags

   ALFA-Tag

   Promoter

   hSyn

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWM_12x601_20bpLinker

   Plasmid

   #157787

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_20bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601-GFP

   Plasmid

   #84040

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with GFP

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTLR4

   Plasmid

   #13085

   Depositor

   Ruslan Medzhitov

   Insert

   TLR4 (Tlr4 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL2-p21 promoter-Luc

   Plasmid

   #33021

   Depositor

   Martin Walsh

   Article

   Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

   Insert

   p21 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   p21

   Available Since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2-2xCLEAR-luciferase

    Plasmid

    #81120

    Purpose

    Reporter activity of the CLEAR network

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    2xCLEAR
    

    Use

    Luciferase

    Tags

    luciferase

    Promoter

    2xCLEAR

    Available Since

    Dec. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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