We narrowed to 4,705 results for: golden gate
-
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL12009
Plasmid#68257PurposeLevel 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)DepositorInsertPromoter and 5UTR Ubiquitin (Zea mays)
UseSynthetic BiologyAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2
Plasmid#182353PurposeGFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK107
Plasmid#110575PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP25 Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)
Plasmid#181791PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mClover3
Plasmid#228624PurposeMammalian expression of mClover3 and extra insert, for MuSIC barcode constructionDepositorInsertmClover3
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterMaize ubiquitin 1Available SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
Plasmid#182351PurposeGermline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK229
Plasmid#110591PurposeBTK Type 3 coding sequence for golden gate assembly and homologous recombinationDepositorInsertKanamycin resistance
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits