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pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

Plasmid

#85452

Purpose

Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-103)

Plasmid

#240597

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-103 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Mutation

"Opt" indicates the I-SceI coding seque…

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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FUS-mCh-sspB

Plasmid

#227377

Purpose

Condensate module, light-inducible oligomerization IDR

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

FUS IDR

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#82400

Purpose

Gateway-compatible zebrafish elavl3 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

elavl3 promoter

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSL103-SpCas9-FLAG-3XNLS

Plasmid

#182032

Purpose

Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

Depositor

Steven Lin

Article

Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

Insert

Streptococcus pyogenes cas9 (cas9 Synthetic)

Use

CRISPR

Tags

12X His tag, 3XNLS, FLAG, and MBP

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKP-GFP

Plasmid

#116940

Purpose

Expression of PFKP-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKP (PFKP Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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