166,289 results

1. PB-CRISPR

   Plasmid

   #160047

   Purpose

   For cloning sgRNA to knockout a specific gene in piggybac plasmid

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_CamKIIa_GFP

   Plasmid

   #96941

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Ricardo Dolmetsch, Oleksandr Shcheglovitov

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   Green fluorescent protein
   

   Use

   Lentiviral

   Promoter

   CaMKIIa

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-25xAARE-minP-EGFP

   Plasmid

   #159666

   Purpose

   EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

   Insert

   25xAARE-minP-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SrtA-PDGFR Tm

   Plasmid

   #121168

   Purpose

   pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   SrtA-PDGFR
   

   Use

   Retroviral

   Tags

   Tomato and flag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX_HA

   Plasmid

   #178534

   Purpose

   Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. VPS13D^EGFP

   Plasmid

   #174109

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   With an internal EGFP tag in aminoacid position 1…

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

   Plasmid

   #217686

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   CELSR3 (CELSR3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-2516

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Mutation

   NGFR truncation: aa 11-276

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-UBC-HLA-E SCT-T2A-GFP

   Plasmid

   #205461

   Purpose

   lentiviral plasmid for expression of HLA-E SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HLA-E SCT (HLA-E Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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