We narrowed to 13,692 results for: Egf

1. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

   Plasmid

   #199350

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NLS-dCas9-HA-NLS--GFP-p300

   Plasmid

   #183925

   Purpose

   p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-p300
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV6-AC 4-μHOM

   Plasmid

   #113619

   Purpose

   Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   4-μHOM variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Use

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-human-pro-IL-18

   Plasmid

   #214317

   Purpose

   Expression of gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-PM-ExRai-AktAR2

   Plasmid

   #184050

   Purpose

   Plasma membrane-targeted ExRai-AktAR2

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   PM-ExRai-AktAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTK331

   Plasmid

   #59572

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(16) 2xK-turn Kozak EGFP attB2 AscI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. G-GECO1-Orai3

   Plasmid

   #73563

   Purpose

   Green fluorescent reporter of Orai3-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1-Orai3 (ORAI3 Human, Synthetic)
   

   Use

   Tags

   G-GECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immdeiate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-PIPK1 gamma 87

    Plasmid

    #22300

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDA095

    Plasmid

    #131391

    Purpose

    generation of Tet-On RPE

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 114_UBI_iCre_crystGFP

    Plasmid

    #171797

    Purpose

    Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised iCre under the control of the Ubiquitin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFRT-TO-DEST

    Plasmid

    #106348

    Purpose

    Destination vector for gateway cloning. Developed for Flp-In T-REx system.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-PKCBII

    Plasmid

    #195232

    Purpose

    Mammalian expression vector containing GFP tagged PKCBII.

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PKCB2 (PRKCB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-mitoT

    Plasmid

    #174519

    Purpose

    Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

    Depositor

    Oleh Khalimonchuk

    Article

    Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

    Insert

    MitoT synthetic tether
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-IRES-GFP-human-Caspase-1

    Plasmid

    #183359

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-E-Syt3

    Plasmid

    #66835

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt3 (ESYT3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. mNeongreen-aGBD(anillin)

    Plasmid

    #129633

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RhoA binding domain of anillin (712-786)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CHMP6_GFP

    Plasmid

    #31806

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP6 (CHMP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. attB-puro-GA donor

    Plasmid

    #182138

    Purpose

    attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVenus-VSVG

    Plasmid

    #11914

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BICDN-CIBN

    Plasmid

    #102253

    Purpose

    Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    BICDN-CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HeLa full-length MLCK-GFP

    Plasmid

    #46316

    Depositor

    Anne Bresnick

    Article

    Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

    Insert

    HeLa long myosin light chain kinase (MYLK Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-Dynamin 2 K44A

    Plasmid

    #22301

    Depositor

    Pietro De Camilli

    Article

    Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

    Insert

    Dynamin 2 K44A (Dnm2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pCIG2-Flag-mNuak1 WT

    Plasmid

    #168507

    Purpose

    For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    Nuak1 (Nuak1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

    Plasmid

    #180587

    Purpose

    FdCd

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CHMP2A_GFP_N_term

    Plasmid

    #31805

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge