We narrowed to 9,972 results for: crispr plasmids

Showing: 7741 - 7760 of 9972 results

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pB006

Plasmid

#182320

Purpose

It's an empty replication plasmid backbone in Bacteroides

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP

Plasmid

#224564

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

Unspecified

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUASTattB-degradALFA

Plasmid

#219663

Purpose

Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

degradALFA

Expression

Insect

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Lv EF1a dCas9-HA 2A Blast

Plasmid

#102812

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

dCas9-HA

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A (dCas9)

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp2x 2A Hygro

Plasmid

#102807

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp2x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pZS165 CRISPEY HH-HDV NotI

Plasmid

#114451

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Expression

Yeast

Promoter

ScGAL7p

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP

Plasmid

#235573

Purpose

Dox-inducible expression of control superfolder (sf)GFP fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

superfolder (sf)fGFP

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-2A-MLH1-SB

Plasmid

#228871

Purpose

Plasmid for MLH1-SB overexpression

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PuroR-2A-MLH1-SB

Use

CRISPR

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PE-Max-pet21A

Plasmid

#204471

Purpose

Expression plasmid for PE protein in bacteria.

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#247356

Purpose

Expresses SpCas9 and a sgRNA targeting the human AAVS1 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

AAVS1 (AAVS1 Human)

Use

CRISPR

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-loxP-STOP-loxP-Venus

Plasmid

#170520

Purpose

Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-loxP-STOP-loxP-Venus

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-PuroR Xlone eGFP

Plasmid

#179837

Purpose

Doxycycline-inducible expression of eGFP

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN ; Donor plas…

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Article

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Insert

scFvGCN4sfGFP-VP64-GB1

Tags

HA and sfGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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cr3Vn

Plasmid

#184861

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, gam, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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