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YFP-ICP0FxE-C3

Plasmid

#134561

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

YFP

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-W3SL

Plasmid

#218744

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_232

Plasmid

#91121

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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Puro.let-7g.Cre

Plasmid

#17409

Purpose

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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PEP109E

Plasmid

#97395

Purpose

binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

Plasmid

#218753

Purpose

Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH*deltavpu/RT-

Plasmid

#136985

Purpose

Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif

Expression

Mammalian

Mutation

carries D110E mutation in the reverse transcripta…

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1-E2C

Plasmid

#180421

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

E2 Crimson

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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5HT2A-APEX2

Plasmid

#215558

Purpose

Stable, inducible expression of 5HT2A

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM60-Cnbp

Plasmid

#38246

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Cnbp (Cnbp Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pIS1-Vim5UTR-renilla

Plasmid

#38237

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim 5'UTR / Renilla (Vim Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Vim 5' UTR fused to renilla ORF

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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