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pU6-hROSA26-1-1kbdonor

Plasmid

#234736

Purpose

PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

hROSA26-1 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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nlsSalicS1

Plasmid

#232190

Purpose

Novel FRET-based biosensor for salicylic acid

Depositor

Alexander Jones

Article

Tang et al Science. 2025 Oct 9;390(6769):188-194. d

Insert

nlsSalicS1

Tags

His-tag

Expression

Plant

Promoter

p16

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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nlsSalicS1-NR

Plasmid

#232192

Purpose

Negative control for nlsSalicS1

Depositor

Alexander Jones

Article

Tang et al Science. 2025 Oct 9;390(6769):188-194. d

Insert

nlsSalicS1-NR

Tags

His-tag and Myc

Expression

Plant

Promoter

P16

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pSUM-GroEL-gfp

Plasmid

#109286

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GroEL-GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

GroEL

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (+1)99xGly

Plasmid

#211347

Purpose

DOX-regulated lentiviral expression of +1 frameshifted 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(+1)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

+1 frameshift to produce polyR frame

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (+2)99xGly

Plasmid

#211348

Purpose

DOX-regulated lentiviral expression of +2 frameshifted 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(+2)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

+2 frameshift to produce polyA frame

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 99xGly

Plasmid

#211356

Purpose

Transient expression of 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly

Plasmid

#211355

Purpose

Transient expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-AAVS1-5-1kbdonor

Plasmid

#234735

Purpose

PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

AAVS1-5 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet3-CAAX-IRES-Puro

Plasmid

#222639

Purpose

Live-cell plasma membrane marker

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mScarlet3-CAAX

Use

Lentiviral

Tags

mScarlet3

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179120

Purpose

Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

control sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TetO_CD19-P2A-mCherry

Plasmid

#133803

Purpose

repressible expression of CD19 and mCherry in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

putative ECD of H. sapiens CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsC

Plasmid

#234732

Purpose

Wild-type human-codon optimized PseTnsC in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsAB

Plasmid

#234731

Purpose

Wild-type human-codon optimized PseTnsAB in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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