Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,030 results for: sgRNA

Showing: 7761 - 7780 of 9030 results

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pLentiCMV-Blast mLST8-Flag Mut2

Plasmid

#124917

Purpose

For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPN067

Plasmid

#91602

Purpose

Express sgRNA targeting human DPP4

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DPP4 (DPP4 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pPN088

Plasmid

#91613

Purpose

Express sgRNA targeting human FURIN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

FURIN (FURIN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCas28

Plasmid

#82385

Purpose

sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23108

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas27

Plasmid

#82384

Purpose

sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23117

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223321

Purpose

It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Inserts

SpCas9 codon optimized for human (cas9 Synthetic, Human)
chloramphenicol resistance protein
CcdB

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en15FnCas9

Plasmid

#201948

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en15FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1603H on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgAFG3L2.1-puro

Plasmid

#257572

Purpose

sgRNA targeting AFG3L2

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

AFG3L2 (AFG3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgAFG3L2.2-puro

Plasmid

#257573

Purpose

sgRNA targeting AFG3L2

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

AFG3L2 (AFG3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgNFU1.1-puro

Plasmid

#257587

Purpose

sgRNA targeting NFU1

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

NFU1 (NFU1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgNFU1.2-puro

Plasmid

#257588

Purpose

sgRNA targeting NFU1

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

NFU1 (NFU1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgHSCB.2-puro

Plasmid

#257582

Purpose

sgRNA targeting HSCB

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

HSCB (HSCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgCHCHD4.1-puro

Plasmid

#257589

Purpose

sgRNA targeting CHCHD4

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

CHCHD4 (CHCHD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgHSCB.1-puro

Plasmid

#257581

Purpose

sgRNA targeting HSCB

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

HSCB (HSCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgGLRX5.1-puro

Plasmid

#257579

Purpose

sgRNA targeting GLRX5

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

GLRX5 (GLRX5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgFECH.2-puro

Plasmid

#257586

Purpose

sgRNA targeting FECH

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

FECH (FECH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgFECH.1-puro

Plasmid

#257585

Purpose

sgRNA targeting FECH

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

FECH (FECH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgISCU.1-puro

Plasmid

#257577

Purpose

sgRNA targeting ISCU

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

ISCU (ISCU Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgSPG7.1-puro

Plasmid

#257574

Purpose

sgRNA targeting SPG7

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

SPG7 (SPG7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgSPG7.2-puro

Plasmid

#257575

Purpose

sgRNA targeting SPG7

Depositor

Kivanc Birsoy

Article

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

SPG7 (SPG7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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Showing: 7761 - 7780 of 9030 results

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