We narrowed to 26,173 results for: STI

1. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMS73

   Plasmid

   #110629

   Purpose

   CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

   Inserts

   short U6 promoter
   cas9
   tnos terminator
   

   Use

   CRISPR; Self-replicating in ustilago maydis, conf…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Bacterial

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U. maydis hsp70 promoter (Kronstad and Leong, 198…

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Syn-Chronos-tdTomato

   Plasmid

   #62726

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

   Plasmid

   #197509

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Expression

   Mutation

   ABE8e mutations in TadA and nSpCas9(D10A)

   Promoter

   Cbh

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

   Plasmid

   #197504

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-ABE8e-NRCH-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

   Plasmid

   #223123

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-PEmax-P2A-EGFP (LM1589)

   Plasmid

   #223136

   Purpose

   pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized PEmax-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

   Expression

   Mammalian

   Mutation

   M-MLV mutations from PE2 (Anzalone et al. Nature …

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Syn-FLEX-rc[Chronos-GFP]

   Plasmid

   #62722

   Purpose

   AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84481

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUW-Flag-Mbd3 delta1-70

    Plasmid

    #52372

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-70

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX311_TRIP13

    Plasmid

    #184538

    Purpose

    Constitutive expression of TRIP13

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Syn-FLEX-fd [Chronos-GFP]

    Plasmid

    #84482

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid

    #122099

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid

    #122103

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid

    #84485

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α, 1.1 kb long (short version)

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge