We narrowed to 1,322 results for: pET28a

1. pET28-NFLtail

   Plasmid

   #232055

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFL tail domain

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Light tail domain
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cDNA codon-optimized for E. coli; added N-termina…

   Promoter

   T7

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-NFMshuf1

   Plasmid

   #232058

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Medium tail domain, charge shuffle 1
   

   Use

   Tags

   6xHis, thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-NFHtail

   Plasmid

   #232057

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFH tail domain

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Heavy tail domain
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   added N-terminal tryptophan and tyrosine, and cys…

   Promoter

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-NFMshuf2

   Plasmid

   #232059

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Medium tail domain, charge shuffle 2
   

   Use

   Tags

   6xHis, thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-28a-YTH domain of YTHDC1

   Plasmid

   #228778

   Purpose

   Protein expression plasmid for YTH domain of YTHDC1 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_LmFPPS

   Plasmid

   #186322

   Purpose

   pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   FPPS
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CpX-1

   Plasmid

   #218676

   Purpose

   Expresses complexin-1 in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   Rat complexin-1 (Cplx1 Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-2H12Matry

   Plasmid

   #221187

   Purpose

   His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen-Matry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_Caspase-11_humanized-mutant

   Plasmid

   #214315

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   N152K_KLS288YKT_KASIHS348TPRAKA

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_Caspase-4_W267N

    Plasmid

    #214314

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    W267N

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_Caspase-4_R269D

    Plasmid

    #214313

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    R269D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL28_Cas9-His Δcys E532C/E1207C

    Plasmid

    #206289

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDL27_Cas9-His Δcys E532C/E945C

    Plasmid

    #206288

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDL26_Cas9-His Δcys M1C/E532C

    Plasmid

    #206287

    Purpose

    Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

    Plasmid

    #191803

    Purpose

    Full length wt AtPCO5 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO5
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ppwd1.473.646.W601A

    Plasmid

    #137660

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Article

    Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

    Insert

    ppwd1 (PPWD1 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    473-646-W601A

    Promoter

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNAP-Kindlin

    Plasmid

    #186136

    Purpose

    For expression of recombinant Snap-labeled kindlin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    FERMT2 (FERMT2 Human)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p40deltaPX (w/out aa1-135)

    Plasmid

    #19019

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa136-148 (PX domain, truncated)

    Promoter

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCpSRb-4

    Plasmid

    #13726

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Circularly permuted SRβD210K183 with Precission p…

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCpSRb-5

    Plasmid

    #13727

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    SRβ circularly permuted D210E188 improved solubil…

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDrSR beta deltaN

    Plasmid

    #13730

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    expresses residues 60-266 of Danio rerio SRβ with…

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-AviTag-DogCatcher-MBP

    Plasmid

    #171928

    Purpose

    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogCatcher-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    DogCatcher added to N-terminus of MBP via a GSGES…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human_TFAM_NoMTS_pET28

    Plasmid

    #34705

    Depositor

    David Chan

    Article

    Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Encodes residues 43–246, corresponding to full-le…

    Promoter

    T7

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PET-28a-YTH domain of YTHDF2

    Plasmid

    #228776

    Purpose

    Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Human USP30 (64-502, construct 8, MG-26-21)

    Plasmid

    #110745

    Purpose

    Bacterial expression of human USP30 (construct 8)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350D, I353E + insertion deletion: 64-357;…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge