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Plasmid#197747PurposePhytobrick (MoClo) Level 0 PartDepositorInsertTaGRF-GIF chimeric protein CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L2-Reverse-Donor-BsaI
Plasmid#197843PurposeDonor vector to restore L2 destination via BsaI (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L2-Reverse-Donor-Esp3I
Plasmid#197844PurposeDonor vector to restore L2 destination via Esp3I (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L1-Reverse-Donor-Esp3I
Plasmid#197845PurposeDonor vector to restore L1 destination via Esp3I (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRA1EGFP
Plasmid#193791PurposeExpresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP RanSen
Plasmid#184697PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)NLS
Plasmid#184689PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen
Plasmid#184691PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen
Plasmid#184700PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-35S-11
Plasmid#176881PurposeMoClo Level 0 plasmid, contains 35SDepositorInsert35S terminator
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(mut)(406)
Plasmid#122069PurposeExpressing a mutant ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationCodon changes without changing the amino acid seq…PromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG7p-ATG1(406)
Plasmid#122071PurposeExpressing ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native promoter was replaced by ATG7 promoter…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(S226A,T228A)(405)
Plasmid#122066PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationSerine 226 to alanine; Threonine 228 to alaninePromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_38J
Plasmid#91154PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4010
Plasmid#91055PurposeModule A, Promoter: ZmUbi, Gene: dTaCas9-VP64, Terminator: HSPDepositorInsertdTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4210
Plasmid#91057PurposeModule A, Promoter: Zmubi, Gene: Csy4-P2A-dTaCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmubiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3801
Plasmid#91053PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dAtCas9-VP64
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_39J
Plasmid#91155PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0302
Plasmid#91006PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0308
Plasmid#91007PurposeModule A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK278
Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK173
Plasmid#71831PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK320
Plasmid#71144PurposeExpression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to serine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK319
Plasmid#71143PurposeExpression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to alanine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK318
Plasmid#71142PurposeExpression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to glutamine; additional N-t…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK314
Plasmid#71140PurposeExpression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to valine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK174
Plasmid#71835PurposeExpression of Acetobacter aceti 1023 PurE and PurK in E. coliDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationadditional N-terminal methionine encoded by this …PromoterT7 and endogenousAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK279
Plasmid#71135PurposeExpression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to aspartate; additional N-t…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK283
Plasmid#71139PurposeExpression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine; additional N-…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK282
Plasmid#71138PurposeExpression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartate; additional N-t…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK281
Plasmid#71137PurposeExpression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to phenylalanine; additional…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK280
Plasmid#71136PurposeExpression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to phenylalanine; additional…PromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only