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Showing: 1901 - 1950 of 2911 results

  8. pEasyG3_nat

    Plasmid
    #184918
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184918-plasmid-map-sequence-id-368408

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  9. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  16. pEA03

    Plasmid
    #172194
    Purpose
    Suicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Jean-Luc Pernodet
    Article
    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:
    Insert
    attR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    172194-plasmid-map-sequence-id-338646

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  23. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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  25. pMflT-o4

    Plasmid
    #101312
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101312-plasmid-map-sequence-id-198865

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  31. pMflPT-o4

    Plasmid
    #101315
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101315-plasmid-map-sequence-id-198861

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  32. pMflST-o4

    Plasmid
    #101316
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101316-plasmid-map-sequence-id-199230

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  34. pP[RS3]3'M

    Plasmid
    #53552
    Purpose
    for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
    Depositor
    Renjie Jiao
    Article
    Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:
    Insert
    AscI and MluI restriction enzyme sites
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    same pP[RS3]
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 and tcp830
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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Showing: 1901 - 1950 of 2911 results