We narrowed to 2,383 results for: 683

1. pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

   Plasmid

   #185683

   Purpose

   Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Human, Synthetic)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   (WLLI-GSGS)[359-362]

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rab11 single guide RNA

   Plasmid

   #229683

   Purpose

   guide RNA for Rab11 tagging at the N-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   Rab11 guide (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_WT RAD23A_delta aa 230-285 (delta XPC-binding domain)

   Plasmid

   #201464

   Purpose

   Expresses a mutant form of human RAD23A that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 230-285

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-LYCHOS (GPR155) ∆DEP-HA

   Plasmid

   #199683

   Purpose

   Transient expression of LYCHOS (GPR155) ∆DEP-HA

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   DEP domain deleted

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-CD1C-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156838

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD1C (CD1C Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-CD1A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156830

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD1A (CD1A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-HLA-G-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156833

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   HLA-G (HLA-G Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. A1-LCD-20G+20S

   Plasmid

   #178683

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-20G+20S (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   twenty Gly residues replaced with Ser

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDC7 gRNA (BRDN0001146695)

   Plasmid

   #76683

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDC7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDC7 (CDC7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-HLA-C-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156831

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-C (HLA-C Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-NTRK2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156839

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NTRK2 (NTRK2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pD649-HAsp-HFE-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156837

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HFE (HFE Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. R703-M80-303: CMV51p> Halotag-Kras4bHVR

    Plasmid

    #159683

    Purpose

    Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Kras4bHVR (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-TSC22D2-Nterm-C-FLAG

    Plasmid

    #222683

    Purpose

    overexpressing N-terminus truncation of TSC22D2 with C-terminal FLAG

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D2 (TSC22D2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    TSC22D2 N-terminus truncation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-HLA-F-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156834

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-F (HLA-F Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pD649-HAsp-BTN2A2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156683

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    BTN2A2 (BTN2A2 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_PTEN_p.G127V

    Plasmid

    #81683

    Purpose

    Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G127V

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCmini-iCAP-AAV9-X1.1

    Plasmid

    #196836

    Purpose

    non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV9-X1.1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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