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We narrowed to 6,937 results for: pcDNA3.1+

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Showing: 761 - 780 of 6937 results

  2. GFP_CD47_LU

    Plasmid
    #65474
    Purpose
    GFP tagged CD47 with its own long 3'UTR
    Depositor
    Christine Mayr
    Publication
    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    Expression
    Mammalian
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65474-plasmid-map-sequence-id-117297

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  6. pcDNA 3.1(-) mouse C/EBP beta (LIP)

    Plasmid
    #12561
    Depositor
    Peter Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)
    Expression
    Mammalian
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles

  7. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Expression
    Mammalian
    Mutation
    D614G
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    177960-plasmid-map-sequence-id-348179

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  9. pCMV-Mm.cargo(Cas9)

    Plasmid
    #174865
    Purpose
    Expresses mouse Peg10 cargoRNA encoding SpCas9
    Depositor
    Feng Zhang
    Publication
    Segel et al Science. 2021 Aug 20;373(6557):882-889.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 flanked by MmPeg10 UTRs
    Tags
    HA-SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174865-plasmid-map-sequence-id-342429

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  11. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  16. GFP-PS9

    Plasmid
    #177944
    Purpose
    Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP (ORF1ab )
    Expression
    Mammalian
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177944-plasmid-map-sequence-id-348205

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  17. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  19. pY30

    Plasmid
    #84745
    Purpose
    Expresses huAsCpf1-P2A-puro and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huAsCpf1
    puromycin resistance gene
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    84745-plasmid-map-sequence-id-162432

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Showing: 761 - 780 of 6937 results