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pHR_CMV_Melt-37-mCherry

Plasmid

#234060

Purpose

Encodes a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-37-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX puro GFP

Plasmid

#250871

Purpose

Transient or stable lentiviral expression of GFP

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

GFP

Use

Lentiviral

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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SCP1-pUC119

Plasmid

#21846

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-pUC119

Use

Puc derivative - a cloning vector

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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GLI2 his

Plasmid

#87097

Purpose

expresses GLI2 in mammalian cells

Depositor

Martin Fernandez-Zapico

Insert

GLI2 (GLI2 Human)

Tags

6x His, T7 tag, and Xpress tag

Expression

Mammalian

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Expression

Bacterial

Promoter

Rhamnose promoter

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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BirA His-tag pLEMO

Plasmid

#119817

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Tags

6-Histidine-tag

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141B

Plasmid

#69291

Purpose

Gateway entry vector; single gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km

Plasmid

#46815

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_PGK

Plasmid

#79120

Purpose

Lentiviral vector for constitutive expression of genes (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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NGFR

Plasmid

#27489

Depositor

Warren Pear

Article

Izon et al Immunity. 2001 Mar . 14(3):253-64.

Type

Empty backbone

Use

Retroviral

Tags

ECMV, IRES, and NGFR

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pHR_AXP_hrGFP

Plasmid

#84613

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3G-tdTomato

Plasmid

#194644

Purpose

Expresses tdTomato under the control of TRE3G

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

TRE3G-tdTomato

Use

AAV

Expression

Mammalian

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-mCherry

Plasmid

#67302

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET his-mCherry

Plasmid

#113552

Purpose

For bacterial expression and purification of mCherry

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mCherry

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Article

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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