We narrowed to 15,732 results for: LEA

1. mTph2p(330bp)-mut3-Luc

   Plasmid

   #223666

   Purpose

   Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 3

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   mTph2 promoter (Tph2 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Promoter

   mTph2 promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTph2p(330bp)-mut4-Luc

   Plasmid

   #223667

   Purpose

   Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 4

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   mTph2 promoter (Tph2 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Promoter

   mTph2 promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

   Plasmid

   #217802

   Purpose

   Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

   Plasmid

   #217806

   Purpose

   Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

   Plasmid

   #217799

   Purpose

   Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS-BCD-Strep-SAVED-CHAT-cbm

   Plasmid

   #214032

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   K121E, Q122A, N274A, R276E, Y285A, F286A

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS-BCD-Strep-dPCaspase

   Plasmid

   #214035

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H79A, C146A

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS-BCD-Strep-PCaspase-R153A

   Plasmid

   #214036

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R153A

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218911

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
   

   Expression

   Mammalian

   Mutation

   2363G>C and 4155C>T and 4729T>G and 4731…

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCDF Bravo-PotH-OL3/2sub

    Plasmid

    #216752

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    potH (potH E. coli (Bacteria))
    

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 2 (OL2) with the sequence of [WMGILKNN…

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid

    #196879

    Purpose

    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1

    Plasmid

    #196892

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1

    Plasmid

    #196891

    Purpose

    Neuron-specific expression of human (h)Centrin fused to 2xHA-tags

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-Nedd1-mOrange2-rCM1

    Plasmid

    #196861

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBactin-Akna-mOrange2

    Plasmid

    #196865

    Purpose

    Expression of the centrosomal protein Akna fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mOrange2 (Akna Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myc-Nix-S35A

    Plasmid

    #197562

    Purpose

    Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-35. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 35 changed to alanine

    Promoter

    CMV

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

    Plasmid

    #196515

    Purpose

    Expression of Myc-Eps8L2_1-496

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_1-496 (EPS8L2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    T7 promoter

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myc-Nix-S35D

    Plasmid

    #197563

    Purpose

    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 35 changed to aspartic acid

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-dMET

    Plasmid

    #176031

    Purpose

    A knock-out vector for dog MET

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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