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pAd5-B6/7deltaE3

Plasmid

#175748

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 25043-35938

Use

Adenoviral and Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(T21N)

Plasmid

#99711

Purpose

Mammalian expression of HA-RagA(T21N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Mutation

T21N

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-biRhoBAST

Plasmid

#196348

Purpose

Expression of mEGFP mRNA with biRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-biRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(Q120L)

Plasmid

#99720

Purpose

Mammalian expression of HA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Mutation

Q120L

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pKD013

Plasmid

#207931

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110.i1

Plasmid

#135481

Purpose

E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110.i1

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
p5954 pcDNA-Xpress-His-USP15 C269A

Plasmid

#23217

Depositor

Peter Howley

Article

Vos et al J Virol. 2009 Sep . 83(17):8885-92.

Insert

USP15 (USP15 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

C269A

Available Since

April 7, 2010

Availability

Academic Institutions and Nonprofits only

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mEos2-H2B-6

Plasmid

#57384

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H2B (H2BC11 Human)

Tags

mEos2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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H2B-LSSmGFP

Plasmid

#186539

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisA-H248Y-Sirt3

Plasmid

#24917

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

His248 mutated to Tyr(Y)

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_CoA-Snifit(V97T)

Plasmid

#197944

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(G41)

Plasmid

#197942

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

Plasmid

#197943

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle mCherry-Tubulin

Plasmid

#26768

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(Q66L)

Plasmid

#99716

Purpose

Mammalian expression of Flag-RagA(Q66L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

Flag

Mutation

Q66L

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFUdioFRTeYFPW

Plasmid

#133998

Purpose

expresses eYFP upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

eYFP

Use

Lentiviral; Flp/frt

Expression

Mammalian

Mutation

inverted and flanked by two incompatible FRT site…

Promoter

UbC

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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