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pENTR-LANP

Plasmid

#16175

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)

Plasmid

#214883

Purpose

Lentiviral vector encoding RfxCas13d targeting PURI guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPURI-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)

Plasmid

#214884

Purpose

Lentiviral vector encoding RfxCas13d targeting GLY guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crGLY-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRS-mEmerald-VIM

Plasmid

#62849

Purpose

Donor vector for gene editing at human VIM locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

mEmerald-VIM (VIM Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSMARCA4 guide 1

Plasmid

#193604

Purpose

SMARCA4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 1 (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-ACE2

Plasmid

#178592

Purpose

Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

Depositor

Ralf Jockers

Article

Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

Insert

angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Flag and SNAP

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_EF1a-NAE1_IRES_Puro

Plasmid

#235659

Purpose

human NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

NAE1 (NAE1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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Nsp14-mCherry

Plasmid

#165137

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-ThymosinBeta4(KK18,19EE)

Plasmid

#96807

Purpose

non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)

Tags

YFP

Expression

Mammalian

Mutation

KK18,19EE prevents G-actin binding

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_13A_3D

Plasmid

#106279

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein and NLS

Expression

Mammalian

Mutation

Deleted amino acids 401-636, T24A, S209A, H212R, …

Promoter

EF1a/RPBSA

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mCherry

Plasmid

#209035

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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RIP7-RLuc-YFP-pBS

Plasmid

#72482

Purpose

Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

Depositor

Vincent Poitout

Article

Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

Inserts

rat ins2 gene promoter (Ins2 Rat)
luciferase
eYFP

Use

Luciferase

Tags

eYFP and renilla luciferase

Expression

Mammalian

Mutation

eYFP varient

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Tags

eYFP

Expression

Mammalian

Promoter

P-TIGHT

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
EGFP-Orf9b L52D

Plasmid

#165129

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf9b L52D

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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