We narrowed to 13,184 results for: BASE
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Plasmid#184371PurposeMammalian expression of nuclease inactive SuperFi-Cas9 CBE base editorDepositorInsertdCBE-SuperFi-Cas9
UseCRISPRTags3X FLAG, SV40 NLS, and UGIExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15543_dABE-SuperFi-Cas9
Plasmid#184373PurposeMammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editorDepositorInsertdABE-SuperFi-Cas9
UseCRISPRTagsFLAG, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-GMRWhite
Plasmid#165885PurposeBlasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 integrase (GB1561)
Plasmid#160586PurposeStreptomyces phage PhiC31 integrase, adapted for C-terminal fusionsDepositorInsertPhiC31
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optop38i5-WPRE
Plasmid#159972Purposeoptogenetic p38 inhibitor with RET to LOV2 to increase sensitivityDepositorInsertmTurquoise2-optop38i5
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optop38i3-WPRE
Plasmid#159971Purposeoptogenetic p38 inhibitor with RET to LOV2 to increase sensitivityDepositorInsertmTurquoise2-optop38i3
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optoJNKi-WPRE
Plasmid#159966Purposeoptogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivityDepositorInsertmTurquoise2-optoJNKi
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAAVExpressionMammalianMutationF434LPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAAVExpressionMammalianMutationV416IPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAAVExpressionMammalianMutationI427VPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-mCherry-C
Plasmid#159437PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertmCherry
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-OgNLuc
Plasmid#135951PurposeExpresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertOgNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH204: pMVP (R4-R3) human PDX1 open
Plasmid#121745PurposepMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertHuman PDX1 (open) (PDX1 Human)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HE302: pMVP (L5-L4) RIP
Plasmid#121728PurposepMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO102: pMVP (R4-R3) eGFP open
Plasmid#121731PurposepMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HJ601: pMVP (R4-R3) mCherry open
Plasmid#121733PurposepMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO601: pMVP (R4-R3) eCFP open
Plasmid#121735PurposepMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only