We narrowed to 15,953 results for: grna

1. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWS5397-CRISPRai-NatR

   Plasmid

   #182836

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB6633

   Plasmid

   #106162

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM134

   Plasmid

   #127215

   Purpose

   BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, IPT, sgRNA
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD5-frame_selector_0,1,2

   Plasmid

   #131152

   Purpose

   Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   CRISPaint frame selector sgRNAs 0,1,2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM119

    Plasmid

    #127213

    Purpose

    BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    GFP, WUS2, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWS5399-CRISPRai-ZeoR

    Plasmid

    #182838

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWS5321-CRISPRai-Markerless

    Plasmid

    #182828

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - markerless

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii, Kunihiko Naito

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM146

    Plasmid

    #127218

    Purpose

    BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, BBM, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM136

    Plasmid

    #127217

    Purpose

    BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, MP-delta, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM135

    Plasmid

    #127216

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, WUS2, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid

    #103867

    Purpose

    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    W85X targeting guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEE391

    Plasmid

    #149281

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_FT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM117

    Plasmid

    #127212

    Purpose

    BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM131

    Plasmid

    #127214

    Purpose

    BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, AtSTM, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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