Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,990 results for: nes

Clear keywords
Showing: 7921 - 7940 of 15990 results

  3. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

    Enlarge

  5. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

    Enlarge

  7. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

    Plasmid
    #242599
    Purpose
    A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)
    Expression
    Mammalian
    Promoter
    human EF1a
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242599-plasmid-map-sequence-id-495380

    Enlarge

  15. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid
    #117319
    Purpose
    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron
    Depositor
    Volker Busskamp
    Article
    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(
    Insert
    hsa-miR-CTRL
    Use
    Transposon-mediated integration
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    miR-124 deleted
    Promoter
    Tet-inducible promoter
    Available Since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    117319-plasmid-map-sequence-id-231738

    Enlarge

  16. pXJ42-p110 CUX1

    Plasmid
    #100814
    Purpose
    Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
    Depositor
    Alain Nepveu
    Article
    Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38.
    Insert
    p110 CUX1 (amino acids 878-1505) (CUX1 Human)
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Contains amino acids 878-1505
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    100814-plasmid-map-sequence-id-205853

    Enlarge

  17. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Article
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 7921 - 7940 of 15990 results