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ABCA1 promoter luciferase reporter (P2 fragment)

Plasmid

#86444

Purpose

Human ABCA1 promoter luciferase reporter (P2 fragment)

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P3 fragment)

Plasmid

#86445

Purpose

Human ABCA1 promoter luciferase reporter (P3 fragment)

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6m.WPRE.SV40

Plasmid

#100841

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h1

Plasmid

#86689

Purpose

For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)

Tags

HA-tag

Promoter

T3

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Promoter

SFFV promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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OPN3-DuET

Plasmid

#213357

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPN3 (OPN3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPAkt

Plasmid

#184053

Purpose

Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-InPAkt

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(Q61L)

Plasmid

#22783

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (Q61L) (RAC1 Human, A. victoria)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 Q61L.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET21a Ov-iPGM

Plasmid

#180245

Purpose

Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Onchocerca volvulus iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Bm-iPGM-bio

Plasmid

#162565

Purpose

Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

B. malayi iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hNPPC-RFP

Plasmid

#22921

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pACT2-CAS9c

Plasmid

#51055

Purpose

Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

Cas9c

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial and Yeast

Promoter

yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-wt-HaloTag

Plasmid

#201551

Purpose

Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1 (partial)

Tags

HaloTag

Expression

Insect

Mutation

Codon-optimized for Drosophila expression

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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