We narrowed to 154 results for: SPT

1. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

   Plasmid

   #227087

   Purpose

   Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   K1355A, K1435A

   Promoter

   T7

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

   Plasmid

   #227088

   Purpose

   Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H, K1355A, K1435A

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

   Plasmid

   #227085

   Purpose

   Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE92 XGCNF-EnR

   Plasmid

   #17029

   Depositor

   Randall Moon

   Article

   Song et al Dev Biol. 1999 Sep 1;213(1):170-9. doi:

   Insert

   XGCNF-EnR (en Fly)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. CAG-EnR

   Plasmid

   #19715

   Depositor

   Connie Cepko

   Article

   Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

   Insert

   engrailed (en Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Drosophila engrailed repressor domain

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLIA EnR (CC#29)

   Plasmid

   #15152

   Depositor

   Connie Cepko

   Article

   Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

   Insert

   Engrailed repressor (en Fly)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   engrailed repressor domain

   Promoter

   Available since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. KHBD00219

   Plasmid

   #34367

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG9015 (en Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TetO-FUW-MCS-EnR_C-term

    Plasmid

    #136885

    Purpose

    Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KHBD00591

    Plasmid

    #39673

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3992 (srp Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid

    #136884

    Purpose

    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p026 pCS2-EnR

    Plasmid

    #11028

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    engrailed (en Fly)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Engrailed repressor domain (bases encoding amino …

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-Brn2-DBD-EnR

    Plasmid

    #19714

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Fly engrailed 36240 (Pou3f2, en Fly, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mouse Brn2 DNA binding domain-Drosophila engraile…

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pET-DEST42-dre4

    Plasmid

    #170440

    Purpose

    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    dre4 (dre4 Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-20

    Promoter

    T7

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. beta2-spectrin-HA

    Plasmid

    #31070

    Depositor

    Vann Bennett

    Insert

    beta2-spectrin (SPTBN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOSGE_CUS1E06

    Plasmid

    #71081

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Spt3 (Spt3 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGEX Supt6h-SH2

    Plasmid

    #46518

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Supt6h (SUPT6H Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1317-1441

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. p029 End-1::EnR

    Plasmid

    #11029

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Dominant inhibitory GATA-binding protein End1::En…

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP4_1BKR

    Plasmid

    #234168

    Purpose

    Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1BKR (SPTBN1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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