Skip to main content
Addgene
Showing: 121 - 160 of 193 results

  1. pBP-Strep(II)

    Plasmid
    #72984
    Purpose
    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Strep(II) N-terminal tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72984-plasmid-map-sequence-id-133833

    Enlarge

  5. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Use
    Tags
    E6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid
    #84299
    Purpose
    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.
    Depositor
    Jonathan Chernoff
    Article
    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71
    Insert
    Mst1 (STK4 Human)
    Use
    Tags
    HA, SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  36. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Use
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

    Enlarge

  38. SBP-ST*-GFP

    Plasmid
    #163668
    Purpose
    Expresses SBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    GFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163668-plasmid-map-sequence-id-322801

    Enlarge

Showing: 121 - 160 of 193 results