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Plasmid#190214PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen2(1-10)
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mNG2(11)
Plasmid#190215PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen2(11)
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mNG2(1-10)
Plasmid#190216PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen2(1-10)
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorInsertCentrin-2 (CETN2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
dsRed-cent2
Plasmid#29523DepositorInsertCent2 (CETN2 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
p6609 MSCV-IP N-HAonly PTENP1
Plasmid#35008DepositorInsertPTENP1 (PTENP1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
PA-DreN
Plasmid#169707PurposeThe optimized DreN246-nMagDepositorInsertDre246-nMag
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F1
Plasmid#184163PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mproSAAS (21 kDa)
Plasmid#231634PurposeMammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)DepositorInsertPcsk1N (Pcsk1n Mouse)
UseTagsExpressionMammalianMutationDeletion of C-terminus (aa181-225)PromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-CETN2
Plasmid#227291PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.DepositorInsertsgRNA Targeting N-terminus of CETN2 (CETN2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET45 His-proSAAS (21 kDa)
Plasmid#231636PurposeBacterial expression of 21 kDa mouse proSAAS chaperone proteinDepositorInsertPcsk1N (Pcsk1n Mouse)
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF3518
Plasmid#144994PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNHLH2 (NHLH2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap TALEN 2
Plasmid#83369PurposePartner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectorsDepositorInsertGAPDH_TALEN2
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-TALEN1
Plasmid#83368PurposePartner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectorsDepositorInsertGAPDH_TALEN1
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Centrin2
Plasmid#136785PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrin2
Plasmid#136784PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-mproSAAS (27 kDa)
Plasmid#231635PurposeMammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.DepositorInsertPcsk1N (Pcsk1n Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Centrin2
Plasmid#136834PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Centrin2
Plasmid#136833PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0902
Plasmid#141751PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPTF1A (PTF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CETN2
Plasmid#227292PurposeDonor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)DepositorInsertCETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
DSPQ-HTT-TALEN1 ZEO
Plasmid#92245PurposeTALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HDDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TPTE_WT
Plasmid#81867PurposeGateway Donor vector containing TPTE , part of the Target Accelerator Plasmid Collection.DepositorInsertTPTE (TPTE Human)
UseGateway entry vectorTagsExpressionMutationL452PPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only