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Plasmid#120278PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorInsertLipin-1 (Lpin1 Mouse)
UseTagsFLAG and in-frame fusion with the delta200 Golgi-…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG02-Amp-FRT
Plasmid#128271PurposePlasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)DepositorInsertTetR-LOV-RRRG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG01-Amp-FRT
Plasmid#128270PurposePlasmid that produces the following fusion protein: TetR-LOV-TIPDepositorInsertTetR-LOV-TIP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKB234
Plasmid#170021PurposeEncodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
hBD3
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG03-AmpFRT
Plasmid#128272PurposeTIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG05-Amp-FRT
Plasmid#128274PurposeTIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG04-AmpFRT
Plasmid#128273PurposeDeg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGfa2(ABDx3)-nLac
Plasmid#53133Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of 3 copies of the gf…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 alpha-actinin 1
Plasmid#11908DepositorInsertalpha-actinin 1 (ACTN1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMEG_BB3_YL68N_AD
Plasmid#117831PurposeGoldenMOCS backbone for 3 TU in Y. lipolyticaDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG8-Amp-FRT
Plasmid#128277PurposeTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFPDepositorInsertsTetR-LOV-TIP
EGFP
KRAS(G12V)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS425ADS
Plasmid#20119DepositorInsertamorphadiene synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron3_sg4_pX458
Plasmid#127339PurposesgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4DepositorInsertMouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterU6 PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124K wgMDH
Plasmid#204258PurposeBacterial expression of R124K wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124KPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124Q wgMDH
Plasmid#204259PurposeBacterial expression of R124Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130E wgMDH
Plasmid#204267PurposeBacterial expression of R130E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196MPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD193EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 I88A wgMDH
Plasmid#204249PurposeBacterial expression of I88A wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationI88APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M66L wgMDH
Plasmid#204247PurposeBacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM66LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M91Q wgMDH
Plasmid#204250PurposeBacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM91QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D87L wgMDH
Plasmid#204248PurposeBacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD87LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 n-myc mPirh2
Plasmid#212994Purposeexpresses mouse PIRH2DepositorInsertpirh2 (Rchy1 Mouse)
UseTagsMYCExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126RPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127PPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123F wgMDH
Plasmid#204255PurposeBacterial expression of P123F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB235
Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBOBI N-FLAG HIPK2
Plasmid#213004Purposeexpresses mouse HIPK2DepositorInsertHIPK2 (Hipk2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8383
Plasmid#126917PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1DepositorInserttHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8382
Plasmid#126916PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtIDepositorInsertXdCrtI
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB6-Lrig1-T2A-tdTomato-FNF-TK
Plasmid#134320PurposeB6J Lrig1-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertLrig1 (Lrig1 Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA::CYP71AV1/CPR
Plasmid#20118DepositorInsertamorphadiene oxidase and p450 reductase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only