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Plasmid#216793PurposeTemplate for in vitro transcription of PEmax.DepositorInsertPEmax
UseTagsExpressionMammalianMutationPromoterT7Available sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS
Plasmid#17362DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
CasRx gRNA targeting EZH2
Plasmid#219655PurposehU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.DepositorInsertCasRx gRNA targeting EZH2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBac{Mhc-453-GFP,attB,w+}
Plasmid#182199PurposeEGFP driven by 453bp enhancer of Mhc geneDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP577
Plasmid#98801PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
UseTagsExpressionBacterialMutationI138T, N171D, N176TPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP971
Plasmid#98805PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV E107D, D127A, S135F, R203Q, K215E
UseTagsExpressionBacterialMutationE107D, D127A, S135F, R203Q, K215EPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP970
Plasmid#98804PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV E107D, D127A, S135F
UseTagsExpressionBacterialMutationE107D, D127A, S135FPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCMK103
Plasmid#226803PurposeIntegrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology armDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK104
Plasmid#226804PurposeIntegrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology armDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK105
Plasmid#226805PurposeIntegrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology armDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK195
Plasmid#226818PurposeIntegrates KanR into NS2, 1250 bp homology, two homologous HIP1 sites in each of the right and left homology armsDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pBac{9Xmef2-eGFP,attB,w+}
Plasmid#182197PurposeEGFP driven by MEF2 transcription factor binding sitesDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{9Xtin-eGFP,w+,attB}
Plasmid#182194PurposeEGFP driven by tinman transcription factor binding sitesDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3Xtin-GAGA-eGFP,w+,attB}
Plasmid#182195PurposeEGFP driven by tinman and GAGA transcription factor binding sitesDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3Xmef2-eGFP,attB,w+}
Plasmid#182196PurposeEGFP driven by MEF2 transcription factor binding sitesDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3Xtin-eGFP,w+,attB}
Plasmid#182193PurposeEGFP driven by tinman transcription factor binding sitesDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH572
Plasmid#172156PurposeNegative control pBAD backboneDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
spoT-
Plasmid#100913Purposedeletes synpcc7942_1377 (spoT)DepositorInsertKanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC
Plasmid#101828Purposedrives expression of kaiBC from an rpaA-independent promoterDepositorInsertkanamycin casette and p0050 promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::glgP_gap1
Plasmid#101845PurposeReplaces native promoter of glgP_gap1 with Ptrc promoterDepositorInsertCmR casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::zwf_fbp_opcA
Plasmid#101846PurposeReplaces native promoter of zwf_fbp_opcA with Ptrc promoterDepositorInsertsp/st resistance casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA
Plasmid#101830Purposedrives expression of rpaA from an rpaA-independent promoterDepositorInsertRpaA with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::gnd
Plasmid#101844Purposedrives expression of gnd using Ptrc promoterDepositorInsertKan casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
zwf-fbp-opcA-
Plasmid#101839Purposedeletes zwf_fbp_opcA locusDepositorInsertKanamycin casette (in place of synpcc7942_2333-2335 operon)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP969
Plasmid#98802PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M
UseTagsExpressionBacterialMutationT146S, D148P, S153N, S170A, N177MPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP824
Plasmid#98803PurposeExpression VectorDepositorInsertpET28 MBP-ENLYGQS-TEV V209M, W211I, M218F
UseTagsExpressionBacterialMutationV209M, W211I, M218FPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVD30
Plasmid#63691PurposepCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.DepositorInsertV. cholera chromosomal DNA
UseTagsExpressionBacterialMutation540 bp of CT A is deletedPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0037
Plasmid#181934PurposeDual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)DepositorInsertsGaussia luciferase
Cypridina luciferase
UseTagsExpressionMammalianMutationPromoterCMV and EF1alphaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR.DT-A
Plasmid#35955DepositorInsertdiphtheria toxin A
UseBac recombineeringTagsExpressionMutationPromoterPGKAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBRα
Plasmid#53731PurposePlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertαNTD
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH625_pJH_LldR_PlldR-sfGFP.0TAG
Plasmid#163724PurposeLactate-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Flag
Plasmid#17364DepositorInsertMMLV proviral DNA
UseTagsFlagExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorTagsExpressionMutationPromoter-Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Forsur-6
Plasmid#188860PurposeAvs inhibitor Forsur-6DepositorInsertForsur-6
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lidtsur-17
Plasmid#188859PurposeAvs inhibitor Lidtsur-17DepositorInsertLidtsur-17
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lidtsur-6
Plasmid#188861PurposeAvs inhibitor Lidtsur-6DepositorInsertLidtsur-6
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPtac
Plasmid#166937PurposePtac single-level gene expression controller (SLC)DepositorInsertGenetic circuit expressing GFP- PTAC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC
Plasmid#166941PurposeDual control based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Dual Control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH25_pBad_AraC_Para-sfGFP.0TAG
Plasmid#163720PurposeArabinose-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertaraC gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB172a
Plasmid#156324PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBSPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH23
Plasmid#107262Purposecontains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.DepositorInsertcurT (slr0483 )
UseSynthetic BiologyTagsmTurquoise2ExpressionBacterialMutationPromoternative CurT promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP972
Plasmid#98806PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
UseTagsExpressionBacterialMutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p64-101
Plasmid#25911DepositorInsertIg germline gamma-1 switch region alpha
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRR5
Plasmid#225095Purposeparental riboswitch reporter vector for regulation of the gfpUV fluorescent proteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotersyntheticAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only