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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,121 results for: LIS;

Showing: 61 - 80 of 30121 results

  1. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Tags
    Expression
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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  3. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  6. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  10. pGD-P1/HC-Pro

    Plasmid
    #196328
    Purpose
    35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tobacco etch virus P1/HC-Pro
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196328-plasmid-map-sequence-id-390012

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  11. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  15. Td-CGBE

    Plasmid
    #196599
    Purpose
    Mammalian expression of Td-CGBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    Use
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196599-plasmid-map-sequence-id-386792

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Showing: 61 - 80 of 30121 results