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Plasmid#112945PurposeProduces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mScarlet-I Fusion
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CytERM-LSS-SGFP2
Plasmid#112940PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LSS-SGFP2
Plasmid#112943PurposeProduces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mCherry fusion
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gaq-LSS-SGFP2
Plasmid#112951PurposeGalphaq tagged with LSS-SGFP2DepositorInsertGalphaq
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-LSS-mEGFP
Plasmid#112942PurposeBacterial expression vector for LSS-EGFPDepositorInsertLSSS-EGFP
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-LSS-SGFP2
Plasmid#112939PurposeBacterial expression vector for LSS-SGFP2DepositorInsertLSS-SGFP2
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-lssmOrange
Plasmid#179795PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertlss mOrange
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSS-SGFP2-aTub
Plasmid#112936PurposeVisualization of microtubulesDepositorInsertalpha tubulin
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
UseTagsExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG2 (GNG2 Human)
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate1
Plasmid#31873DepositorInsertNLS-LSS-mKate1
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PRLSS-SH2-KDEL
Plasmid#209716PurposeMammalian expression of SH2 localized to endoplasmic reticulumDepositorInsertPRLSS-SH2-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-T2A-LSS-SGFP2
Plasmid#112946PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmScarlet-I and LSS-SGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-T2A-LSS-SGFP2
Plasmid#112944PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmCherry and LSS-SGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-mScarlet-i
Plasmid#191199PurposeFusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-SAV
Plasmid#191200PurposeFusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
Plasmid#207159PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG1
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only