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We narrowed to 5,045 results for: golden gate

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Showing: 61 - 80 of 5045 results

  3. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Expression
    Plant
    Promoter
    U6 composite
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

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  8. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

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  9. pTALEN(Nd154C63) SFFV

    Plasmid
    #50431
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50431-plasmid-map-sequence-id-75352

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  10. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  11. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

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  12. pTALEN(Nd154C63) MND

    Plasmid
    #50432
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    MND
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50432-plasmid-map-sequence-id-75353

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  13. pTALEN(Nd154C63) Ef1a

    Plasmid
    #50433
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50433-plasmid-map-sequence-id-75354

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  14. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35188-plasmid-map-sequence-id-42839

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  15. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35189-plasmid-map-sequence-id-68899

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  16. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40788-plasmid-map-sequence-id-68904

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  17. pICSL12013

    Plasmid
    #245630
    Purpose
    Level 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    6GAL4UAS promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245630-plasmid-map-sequence-id-495460

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  18. pICSL50020

    Plasmid
    #245582
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag eCFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245582-plasmid-map-sequence-id-495505

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  19. pICSL50068

    Plasmid
    #245623
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag mTurquoise2 (monomeric)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245623-plasmid-map-sequence-id-495482

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  20. pICSL13003

    Plasmid
    #245653
    Purpose
    Level 0 Golden Gate part 6GAL4UAS (Dex inducible expression system) promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    6GAL4UAS (Dex inducible expression system) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245653-plasmid-map-sequence-id-495394

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Showing: 61 - 80 of 5045 results