We narrowed to 3,764 results for: golden gate

1. p367_DG

   Plasmid

   #234689

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Tags

   2X Strep tag and His10-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p362_DG

   Plasmid

   #234684

   Purpose

   Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-Opsin

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Tags

   His6-3C-3XFLAG-SUMO and Thrombin-Opsin

   Expression

   Bacterial

   Mutation

   No

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNDGG039

   Plasmid

   #231339

   Purpose

   Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Expression

   Bacterial

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNDGG038

   Plasmid

   #231338

   Purpose

   Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Expression

   Bacterial

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNDGG037

   Plasmid

   #231337

   Purpose

   Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
   

   Expression

   Bacterial

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L0I75_tUBQ10_GG5-GG6

   Plasmid

   #219714

   Purpose

   Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG5-tUBQ10-GG6
   

   Use

   Synthetic Biology

   Mutation

   WT terminator with added golden gate linkers

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOGG026

   Plasmid

   #113992

   Purpose

   Level 1 golden gate cloning vector for low copy number stable environmental gene expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOGG024

   Plasmid

   #113991

   Purpose

   Level 1 golden gate cloning vector for medium copy number free-living expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOGG216

   Plasmid

   #114002

   Purpose

   Level 2 golden gate cloning vector for medium copy number free-living expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOGG042

    Plasmid

    #113998

    Purpose

    Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Tetracycline
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOGG009

    Plasmid

    #113983

    Purpose

    Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Gentamicin
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGG008

    Plasmid

    #113982

    Purpose

    Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Kanamycin/neomycin
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. L0I89-pMUTE_GG0-1

    Plasmid

    #219708

    Purpose

    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pMUTE-GG1
    

    Use

    Synthetic Biology

    Mutation

    WT promoter with added golden gate linkers

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L0I74-_tUBQ10_GG4-GG6

    Plasmid

    #219713

    Purpose

    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Mutation

    WT terminator with added golden gate linkers

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L0I87-SPCHPromoter_GG0-1

    Plasmid

    #219707

    Purpose

    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH-GG1
    

    Use

    Synthetic Biology

    Mutation

    WT promoter with added golden gate linkers

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L0I84 - GG0-pAHP6-GG1

    Plasmid

    #219706

    Purpose

    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pAHP6-GG1
    

    Use

    Synthetic Biology

    Mutation

    WT promoter with added golden gate linkers

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTALE64G

    Plasmid

    #35188

    Purpose

    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition

    Depositor

    Gang Bao

    Insert

    TAL Effector-VP64-T2A-eGFP
    

    Use

    TALEN

    Expression

    Mammalian

    Mutation

    +63 C Terminus, VP64 Domain, T2A-GFP

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pTALE64

    Plasmid

    #35189

    Purpose

    Golden Gate Compatible TALE Transcription Factor using VP64 Domain

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Transcription Factor
    

    Use

    TALEN

    Expression

    Mammalian

    Mutation

    +63 C Terminus, VP64 Domain

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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