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pX330A-Prdm14-L3-#2

Plasmid

#171510

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Prdm14-L4-#1

Plasmid

#171511

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Prdm14-L4-#2

Plasmid

#171512

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-10xSunTag (SRv1.1)

Plasmid

#185798

Purpose

pcp-10xSunTag component for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-10xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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ubc-nls-pcp-12xSunTag (SRv1.2)

Plasmid

#185799

Purpose

pcp-12xSunTag component for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-12xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V

Plasmid

#63586

Purpose

psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

Depositor

David Rawlings, Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Packaging plasmid for integrase deficient lentiviral vectors

Use

Integrase deficient lentiviral packaging vector

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

Plasmid

#31482

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce Target) EF Puro

Use

Lentiviral

Mutation

mCherry has M9S and M16L mutations. This is what …

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PGK plasmid

Plasmid

#169744

Purpose

Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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Dead-Laconic/pcDNA3.1(-)

Plasmid

#118627

Purpose

To express in mammalian cells a lactate FRET sensor negative control.

Depositor

Luis Felipe Barros

Article

Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

Insert

Dead-Laconic

Expression

Mammalian

Mutation

Change Histidine 151 to Aspartic Acid of Escheric…

Promoter

CMV Promoter

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Article

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

Plasmid

#231601

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

Plasmid

#231602

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

Plasmid

#231594

Purpose

Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Kozak sequence-Tornado-10xSunTag-2xALFATag

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

Plasmid

#31477

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 T2A mTagBFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y + S111Y on I-AniI = Y2 "reo" (res…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
CMV+ plasmid with onboard mCherry cassette

Plasmid

#169741

Purpose

CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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